英国ANGLE公司的Parsortix系统采用专利的微流控技术，使用微流控芯片，捕获和收集循环肿瘤细胞（CTC）。基于CTCs与全血中其他血细胞的不同特性（CTCs具有可变形性差、尺寸大的特性），来捕获CTCs。

捕获的CTCs可以在微流控芯片中进行固定和染色，可直接在显微镜下进行鉴定和计数，或者收集到细胞培养管中，用于下游实验分析，比如：细胞培养、高通量测序、表型分析、药物筛查等。

* 可以捕获和收获多种癌症的CTCs，包括前列腺癌、乳腺癌、肺癌、结肠癌、直肠癌、肾癌和卵巢癌等。
       * 捕获CTCs不需要使用抗体（非免疫方法捕获），某些肿瘤标记物表达量低的CTCs也可以被有效捕捉到（比如卵巢癌细胞、肝癌细胞、黑色素瘤细胞等）。
       * 全血上样体积可高达20ml。
       * 操作简单，人为干预少，无需对血液样本进行预处理。
       * CTCs染色方案可定制，可直接在芯片中染色，最多可做6种颜色的染色。
       * 只需100微升缓冲液，即可回收CTCs，为下游实验做好准备。

1. 分离和收集患者血液样本中CTCs
2. 从患者血液中采集的CTCs， 可以进行多组学分析

Effect of short-term storage of blood samples on gene expression in lung cancer patients. Medical University of Vienna, Vienna,
Austria, published by Clinical Chemistry and Laboratory Medicine, November 2022
The RPL/RPS Gene Signature of Melanoma updates CTCs Associates with Brain Metastasis. University of New Mexico Health
Sciences Center,Albuquerque, New Mexico, USA, published by Cancer Research Communications, November 2022
Characterization of RARRES1 Expression on Circulating Tumor Cells as Unfavorable Prognostic Marker in Resected Pancreatic Ductal
Adenocarcinoma Patients. University Hospital Hamburg-Eppendorf, Hamburg, Germany, published by Cancers, September 2022
A Multi-Center Clinical Study to Harvest and Characterize Circulating Tumor Cells from Patients with Metastatic Breast Cancer Using
the Parsortix® PC1 System.University of Texas MD Anderson Cancer Center, Houston, Texas, USA; Wilmot Cancer Institute, University of Rochester
Medical Center, Rochester, New York, USA; Robert H Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois,
USA; Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California, USA , published by Cancers,
October 2022
Comparative analysis of capture methods for genomic profiling of circulating tumor cells in colorectal cancer. Universidade de Vigo,
Vigo, Spain and the Galicia Sur Health Research Institute , Spain, published by Genomics, October 2022
Comparison of microfuidic platforms for the enrichment of circulating tumor cells in breast cancer patients. Department of Obstetrics
and Gynecology, Comprehensive Cancer Center, Medical University of Vienna, Vienna, Austria, published by Breast Cancer Research
and Treatment, September 2022
Characterizing the epithelial–mesenchymal transition status of circulating tumor cells in head and neck squamous cell carcinoma.
Institute of Head and Neck Studies and Education, Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham,
United Kingdom, published by Head and Neck, July 2022
Malignancy Assessment Using Gene Identification in Captured Cells Algorithm for the Prediction of Malignancy in Women With a
Pelvic Mass Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Wilmot Cancer Institute, University of
Rochester, Rochester, New York and ANGLE Europe Limited, Surrey Research Park, Guildford, Surrey, United Kingdom, published by
Obstetrics & Gynecology, September 2022
Powering single cell genomics to unravel circulating tumour cell subpopulations in non small cell lung cancer patients. Edith Cowan
University, Perth, Australia, published by Journal of Cancer Research and Clinical Oncology, July 2022
Implementing microwell slides for detection and isolation of single circulating tumor cells from complex cell suspensions. University
Hospital and Medical Faculty, Heinrich-Heine University Dusseldorf, Dusseldorf, Germany, published by Cytometry, June 2022
Metabolism-Related Gene Expression in Circulating Tumor Cells from Patients with Early Stage Non-Small Cell Lung Cancer. National
and Kapodistrian University of Athens, Greece, published by Cancers, June 2022
DNA methylation analysis of tumor suppressor genes in liquid biopsy components of early stage NSCLC: a promising tool for early
detection National and Kapodistrian University of Athens, Greece, published by Clinical Epigenetics, May 2022
Analysis of Single Circulating Tumor Cells in Renal Cell Carcinoma Reveals Phenotypic Heterogeneity and Genomic Alterations Related
to Progression. Fondazione IRCCS Istituto Nazionale Tumori, Milano, Italy published by International Journal of Molecular Sciences,
February 2020
Pre-Analytical and Analytical Variables of Label-Independent Enrichment and Automated Detection of Circulating Tumor Cells in
Cancer Patients .University Medical Center Hamburg-Eppendorf, Hamburg, Germany published by Cancers, February 2020
Detection and prognostic role of heterogeneous populations of melanoma circulating tumour cells. Edith Cowan University, Perth,
Australia published by British Journal of Cancer, February 2020
Intra-Patient Heterogeneity of Circulating Tumor Cells and Circulating Tumor DNA in Blood of Melanoma Patients. University Medical
Center Hamburg-Eppendorf, Hamburg, Germany published by Cancers, October 2019
Circulating Tumour Cell Biomarkers in Head and Neck Cancer: Current Progress and Future Prospects. Institute of Head and Neck
Studies and Institute of Immunology and Immunotherapy, University of Birmingham, UK published by Cancers, August 2019