A conventional cell sorter gives damage to separated cells as electric charge and collision and the damage causes great inconvenience to study the performance and characteristic of the cells. Moreover, It is impossible to perform selection of sheath liquid and sorting of the cell by various culture medium in conventional sorting.

According to the disadvantages of traditional flow cytometry ,On-chip Biotechnology developed a new type of instrument in 2012 which having great feature with easy controlling and maintenance-free, damage-free and contamination-free .The On-chip cytometer uses small pressure , even sensitive cells like neuronal cells, etc. can be sorted without damage.

In addition, this oringinal the cell sorter adopted disposable micro fluidic chips, so it can separate precious samples without any damage and loss to solve that problem and cross contamination will not arise either. Because of the disposable exchangeable micro chip , the instrument is maintenance -free. Without washing fluidic cycle ,it is simple and convenient to start using it immediately.

Using disposable exchangeable microfluidic-chip module

No requirement for sheath liquid (could use the sea water , culture, etc.)

Damage-free sorting

contamination-free (could be installed in the biosafety cabinet)

Upgrade to 3 lasers and 6 PMTs（405nm,488nm,561nm,637nm,785nm）

Simply operation, calibration-free for optical path and flow system

maintenance-free

Upgrade from On-chip Flow to On-chip Sort

Include, but are not limited to

l CTCs detection and separation

l Nerve cells sorting

l Sperm sorting

l Parasite sorting

l Regenerative cells (ES cells,iPS)

l Algae identification and separation

Other Conventional applications:

l Bacterial viability

l Cell cycle

l Apoptosis

l Side population detection

l Immunophenotyping

.....

Applications for sorting

1 Rat hippocampus cells

Hippocampus cells from rat embryo were taken and sorted by a conventional droplet sorter and On-chip Sort. Right after sorting ,cells were stained by PI to check viability.Rest of the cells were kept culture up to 7days.

Cell morphology show clear difference between cells sorted by Sorter A and On-chip Sort ,as early as day3.Cells sorted by Sorter A did not survive whole cells sorted by On-chip Sort showed strong extension of axis.

2 Sperm

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2 Jin Akagi, Magdalena Kordon, Real-time cell viability assays using a new anthracycline derivative DRAQ7®Cytometry A. 2013 February ; 83(2): 227–234.

3 Joanna Skommer, Jin Akagi, .Multiparameter Lab-on-a-Chip flow cytometry of the cell cycle. Biosensors & bioelectronics 11/2012; 42C:586-591

4 Jin Akagi, Kazuo Takeda, .Microflow Cytometry in Studies of Programmed Tumour Cell Death.Procedia Engineering 02/2012; 47:88–91.

5 Susumu Yoshie, Jun Ito. Establishment of novel detection system for embryonic stem cell-derived hepatocyte-like cells based on nongenetic manipulation with indocyanine green.Tissue Engineering Part C Methods 09/2011; 18(1):12-20

6 Fumie Jimma, Yuu Fujimura and Kazuo Takeda ;Small-scale cell analysis by the microfluidic flow cytometer, FISHMAN-R, using a disposable chip.Cytometry Research. 03/2011; 21(1):31-36.

7 Kazuo Takeda, Yu Fujimura and Fumie Jimma;Development of a new compact cytometer using a disposable microfluidic chip for contamination-free and biosafety measurement.Cytometry Research. 03/2011; 21(1):43-50.

8 Masashi Takao and Kazuo Takeda; Enumeration, characterization, and collection of intact circulating tumor cells by cross contamination-free flow cytometry;
Cytometry A. 79(2),93-4, 2011

9 Que Chi Truong-Bolduc, Gilles R. Bolduc. Implication of the NorB Efflux Pump in the Adaptation of Staphylococcus aureus to Growth at Acid pH and in Resistance to Moxifloxacin.ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, July 2011, p. 3214–3219

10 Ken Nishimura, Masayuki Sano, Manami Ohtaka. Development of Defective and Persistent Sendai Virus Vector: a Unique Gene Delivery/Expression System Ideal for Cell Reprogramming;J. Biol. Chem. 2011, 286:4760-4771.

11 Kazuo Takeda and Fumie Jimma; Maintenance free biosafety flow cytometer using disposable microfluidic chip (FISHMAN-R);Cytometry B. 76B 405-406. 2009